Translator for HPLC HINTS and TIPS for Chromatographers

Saturday, December 8, 2018

Mobile Phase Preparation; Part 1, Overlooked HPLC Chromatography Standard Operating Procedures (SOP's)

As a scientific consultant, I often review company overall laboratory operations and make recommendations regarding documentation and procedures which may improve their accuracy and results. Some of these recommendations come in the form of SOP's.

Here is the first example (of three installment posts) of a 'must have' SOP' which should be in place for any laboratory performing HPLC analysis.

Part 1 :  
Procedure(s) For the Preparation of MOBILE PHASE :

Proper documentation of HPLC methods shall always include all of the information that someone would need to reliably reproduce the method in another laboratory. This includes the instrument brand, model, module numbers, configuration, details of the column type with the dimensions and particle size, flow rate, mobile phase composition, all detection parameters including flow cell dimensions, path length/volume, wavelength(s) and bandwidth (if applicable), sampling rate, injection solution, injection volume, sample concentration and other critical information. 
  • An area which is often overlooked is HOW the mobile phase solutions are prepared. In addition to stating the chemical grades used, pH measurement checks/adjustments and if any filtering is required, mobile phase preparation often include weighing, dispensing and mixing steps, each of which needs to be described in detail if they are to be reproduced. Without clear directions, the composition of the mobile phase may be different. For example, do you weigh or measure (volume) out all liquids? What type of glassware are used to measure volumes? Volumetric flasks, beakers, graduated cylinders (and if so, what tolerance grade or class are they?) When mixing two solutions, do you measure and prepare them separately in two containers (if so, which containers?), then mix them (how do you mix them)? Do you fill one container with one liquid, then fill to the desired level with the second one? Do you need to check the pH of the solution (as well as how to adjust the pH of the solution? With what?)? When and how? Do you have a SOP for the pH meter and how/when to calibrate it? Number of standards used (usually 3 are used)? Is the final solution filtered, and if so, by what method (be specific)? I have seen people use different methods each time they prepare a solution. As you can see, each procedure results in a final composition which will be different. Different mobile phase compositions usually lead to different results. The important message here is to use the SAME method to prepare solutions and to document it in a SOP for the method (and for all methods). Additionally, be sure there is formal training to insure that everyone prepares solutions in the same manner. Most labs will need multiple SOP's for mobile phase preparation, but as a general guideline, you should have one master SOP for the preparation of mobile phase solutions. The SOP should also include detailed information regarding STORAGE time limits for each type.This will set the standard from which the other SOP's can be based on.
  • Be sure to prepare FRESH, filtered aqueous mobile phase solution for use each day. Prepare only enough solution for one or two days use (refrigerate the solution to get 2 days), then dispose of any remaining solution and start with fresh solution. Do not store aqueous solutions at room temperature or for extended periods.
  • Some types of ORGANIC solvents may require special SOP notes regarding preparation (e.g. pre-filtering), stabilization additives (e.g. mol sieves, dry Nitrogen gas, etc), types of storage (e.g. amber glass bottles away from light), expiration dates after opening (e.g. Acetic Acid, DEA, TEA, THF, etc). Be sure to include these in your SOPs.

Notes on SOP creation and editing: Make sure you have several people review the draft SOP's before approving. Sometimes what appears clear to you may in fact have a different meaning to someone else. Clear laboratory procedures should contain enough detail that people with different backgrounds will each carry out the procedure in the same manner. Often, these types of documents will go through many drafts and even after approved, should also be open to future suggestions to make them even better.

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PART #2 of this three-part series, "HPLC System Preventative Maintenance Frequency & Procedure (PM); Part 2, Overlooked HPLC Chromatography Standard Operating Procedures (SOP's)" can be found at this link:

4 comments:

  1. Great ! We have been experiencing a problem with method reproducibility after transferring it to another lab which has the same HPLC system. After reading one of your other articles we realized how important all of the instrument settings and options are in reproducing methods.

    We were able to correct all of the Method transfer problems we had, after using the knowledge gained from this HPLC blog. For example - Someone at the other lab read a few of your posts which mentioned how the reference wavelength software feature can change the data. The method failed after transfer and the reasons and one of the reasons was that they had been using the manufacturer's default settings (instead of actual settings) for the Reference Wavelength. Now, they know better and always turn it OFF. Another problem was caused by them using a flow cell which had different dimensions than the one we used (6mm vs 10mm path length). And another problem was that they prepared mobile phase differently than we do. This problem was the fault of both users and neither one of us documented in detail the mobile phase preparation (with an SOP as you suggested). Now, both of us use SOP's to document the preparation of ALL mobile phases and ALL methods in our lab.

    Everyone has learned so much from these articles!!! You are the HPLC EXPERT for sure.

    ReplyDelete
    Replies
    1. Thank you for your comments and success story. Learning does not stop outside of the classroom. We must all continue to ask questions and refer to the basic fundamentals when troubleshooting.

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  2. I am facing a problem. When i am running 80(methanol):20(buffer). Hplc pressure fluctuates a lot and it came down to 20-30 psi.

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    Replies
    1. Please review the article, "Diagnosing & Troubleshooting HPLC Pressure Fluctuation Problems (Unstable Baseline)" to find the solution and be sure to contact someone experienced in HPLC operation to assist you.

      https://hplctips.blogspot.com/2014/01/diagnosing-troubleshooting-hplc.html

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