Translator for HPLC HINTS and TIPS for Chromatographers

Saturday, October 7, 2017

Preparation of Phosphate Buffered Saline (PBS)



PBS



While not commonly used in liquid chromatography, PBS solution is commonly used in preparing samples. By popular request, I am provided a common laboratory recipe for the solution here.


To make Phosphate Buffered Saline (PBS) solution:


Method #1:

1. To a 1-liter flask, add the following four anhydrous salts:

a. 200 mg KCI

b. 8,000 mg NaCI

c. 200 mg KH2PO4

d. 150 mg Na2HPO4

2. Add about 850 ml of deionized water and stir to dissolve the salts. When fully dissolved, fill to the “line” with more deionized water. Stir a final time to insure a uniformly mixed solution.

3. Pour the contents into a laboratory beaker and adjust the pH to 7.0 with 10% phosphoric acid (phosphate solutions should be adjusted with phosphoric acid only).
4. Filter the final solution through a suitable 0.22 micron filter before use.




Method #2:

Optionally, use the same ingredients as specified above, but premix in a beaker with stir bar to make the job easier. 

Place a 1 L laboratory glass beaker on a hotplate stir with stir bar. Fill the beaker with 850 ml of deionized water. Stir at a moderate rate with some heating (~ 35C). Add the dry ingredients to the solution and allow time for them to dissolve. When fully dissolved, remove the stir bar, remove from the heat and carefully pour the contents into a 1 L volumetric flask. Allow the solution to cool to ~ 20C. Fill to the line with deionized water, stopper and mix the final solution (inversion). Pour the contents back into a laboratory beaker and adjust the pH to 7.0 with 10% phosphoric acid (phosphate solutions should be adjusted with phosphoric acid only). 


Filter the final solution through a suitable 0.22 micron filter before use.

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