Windows 7 Shortcuts & Tips

Here are some of My Favorite Helpful Shortcuts/Tips For Use With Microsoft Windows 7.0 

  Do you miss the 'Quick Launch Bar' ? The bar is absent in Windows 7, but it can be put back.

1.   Use your mouse to right click on the Windows taskbar and then choose Toolbars; New Toolbar.
2.   The system will ask you for the path of the new folder. Type:   %userprofile%\AppData\Roaming\Microsoft\Internet Explorer\Quick Launch
3.   Select 'folder'. A new link to the quick launch bar will be added to your current task bar. 

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  Are you using a laptop and want to know how the power is being used ? There is a built-in application which will create a file containing a power efficiency report for you.

1. From the command line type ("Folder" is the name you supply where you want the report to go): powercfg -energy -output \Folder\Energy_Report.html

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  Would you like to minimize all of the running application windows in one shot instead of minimizing them one at a time ? Windows 7 has a feature for this called " shake". The window that you are currently using will stay active and the rest will be minimized. Here is how to use it.

1. Click and hold the title bar of the current active window you want to stay on-top. 
2. While holding it with the mouse, shake the item back and forth until all of the other windows are minimized. 
3. Once they are, let go of the mouse button. 
4. Shake the title bar once again to bring them all back.

Hydrophilic Interaction Chromatography (HILIC)

Perhaps you have a polar sample which shows poor or no retention under reverse phase conditions. HILIC may provide you with an alternative method for retention and separation. HILIC is a unique mode of chromatography which uses numerous retention mechanisms. The most important mechanisms involve surface layer liquid-liquid partitioning, adsorption and various types of ionic interactions.

Sometimes referred to as "aqueous normal phase chromatography", this hybrid technique utilizes a stationary phase which is very polar (e.g. silica, amino or a diol column) and a mobile phase which is made up mostly of organic phase with some water added. The retention mechanism is based on the idea that adding a low percentage of polar phase (water in this case) to a polar surface will result in a water layer forming. Typically this hydrophilic layer results when as little as 2 or 3% water is added to the mobile phase. The remainder of the mobile phase is an organic solvent (ACN is the most popular, but many others can be used). The polar charged analyte(s) will partition into and out of this adsorbed water layer (often, a cation exchange process takes place, but their may be a purely electrostatic mechanism going on as well). Unlike conventional reverse-phase chromatography, in HILIC increasing the organic content of the mobile phase increases the retention! Put another way, increasing the water content of the mobile phase and decreasing the organic portion (as in an HILIC gradient method) results in retention and then elution of very polar analytes. 

With the HILIC mode, sample elution (retention) decreases as you increase the polarity of the organic solvent. Based on this information, good HILIC column wash solutions usually use alcohols in place of ACN  (IPA, Ethanol and Methanol; with Methanol being a stronger eluter). For best results, consider incorporating an alcohol wash after each analysis. Allow plenty of time for the column to equilibrate too.

As with other modes of chromatography, the use of additives, buffers and pH can all play a role in retention and separation plus improve reproducibility. When developing methods, be sure and evaluate their role. Because of the low water content of most methods, buffers must be chosen carefully to insure full solubility. Ammonium formate and acetate are popular as are acids such as formic acid. Regarding pH, the low aqueous portion will mean that the actual pH of the final solution will be much closer to neutral.

 

• Caution: Sales and marketing people sometimes stick an HILIC label on an existing silica column to create a new product. No "special" HILIC columns are needed to develop an HILIC method. Since HILIC is a mode of chromatography, not a support type, most any high quality, NP silica column can be used.

 

As some HILIC methods may be hard to reproduce (very sensitive to changes in composition and long equil times) they are best used by more experienced cinematographers, only after conventional methods have been unsuccessful.

Techniques To Enhance Negative Mode ESI in LC/MS

Many sample types require MS analysis using negative ion electrospray mass spectrometry (ES MS) mode. Sensitivity can be less in this mode, compared to positive mode, as they employ mostly non-polar solvents which do not promote ion formation. In the past we discussed how adduct formation can be employed to enhance ion formation. There are other techniques which can be used as well. Here are a few tips which can be used to improve the quality of the signal obtained under negative mode ES MS conditions.

(1) Negative ion formation and signal response can be improved by choosing the right solution chemistry. One method to improve solvent desolvation and reduce the electrical discharge (noise) is to add isopropyl alcohol (2-Propanol) to the mobile phase. Reported in the literature nearly twenty years ago, as little as a 10% solution has been found to increase the signal level and decrease the noise level under many conditions. The addition of methanol to the mobile phase also can improve the signal, though usually not to the same degree as IPA.


(2) Another technique used to improve ionization involves the pH of the solution or mobile phase used. Higher pH values are often better. Post column addition of a basic solution which adds proton acceptors to the mix, such as ammonium hydroxide (20 or 30 mM), can also improve signal response and stability.


(3) APCI mode: If running nonpolar to semi-polar small molecular weight compounds, especially those which are volatile, this is still the source of choice. *Conventional ESI mode is best for polar to neutral molecules in small to large Mw ranges.

Popular Microsoft Windows® Short Cuts:

Here is a short list of some of the most popular Windows short cuts. Hopefully, you will spot a few that you do not know about and can use.

• Windows Keyboard Logo Symbol + L: Lock the computer (without using CTRL+ALT+DELETE).

• ALT+TAB: Easily switch between open programs.
• ALT+F4: Quit the program.
• ALT+F4: Closes the current window.

• SHIFT+DELETE: Delete an item permanently.
• SHIFT: Press and hold down the SHIFT key while you insert a CD-ROM to bypass the automatic-run feature.

• CTRL+ESC: Open Start menu.
• CTRL+C: Copy.
• CTRL+X: Cut.
• CTRL+V: Paste.
• CTRL+Z: Undo.
• CTRL+B: Bold Font.
• CTRL+U: Underline Font.
• CTRL+I: Italic Font.
• CTRL+Z: Undo the last command

A Full List of Microsoft Short Cut Commands Can Be Found In This Key Directory (Listed by Application): http://support.microsoft.com/search/default.aspx?query=Microsoft%20shortcut%20commands&catalog=LCID%3D1033