HPLC CHROMATOGRAPHY HINTS and TIPS FOR CHROMATOGRAPHERS [HPLC TRAINING ARTICLES]

The HPLC "Hints and Tips" found here will be of interest to anyone performing HPLC, UHPLC (UPLC), LC/MS, SFC, CE or GC analysis. Many of these free tips, taken from professional classes that I teach, discuss fundamental to advanced level chromatography concepts in a practical way. Occasionally, I will also include laboratory computer related tips here too. Please "follow" this blog to receive updates of new posts. Hint: Type keywords into the SEARCH bar to quickly find other articles.

Showing posts with label Troubleshooting. Show all posts
Showing posts with label Troubleshooting. Show all posts
Saturday, April 6, 2024

Troubleshooting a GRADUAL HPLC PUMP PRESSURE INCREASE OVER TIME (When PURGING, DURING an ANALYSIS or when FLOW is DIRECTED TO WASTE)

›
A fully equilibrated column at a constant temperature and flow rate should result in a stable back-pressure over time (~1% variation). Have ...
Saturday, August 21, 2021

Troubleshooting HPLC Gradient Valve / Proportioning Valve / MCGV GPV Leaks. How to Identify Them.

›
HPLC pumps which utilize low-pressure mixing VALVES are known by names such as: "Ternary" (3-solvents) or "Quaternary"...
3 comments:
Saturday, October 31, 2020

Capillary Electrophoresis (CE) Troubleshooting Tips:

›
What follows is a short list of problems, "observations" followed by a list of areas that should be investigated, as appropriate i...
Saturday, September 5, 2020

Tips and Advice for Priming your HPLC PUMP (or similar pumps, FPLC or UHPLC Pump)

›
The single most important component of any HPLC system is the Pump module. We often refer to it as "the heart of the HPLC system...
3 comments:
›
Home
View web version

About Me

HPLC EXPERT
AKA: "The HPLC Doctor". An expert Chromatographer, Scientist, Consultant and Inventor who works in the Analytical, BioMedical, BioPharma, Medicinal Chemistry, Medical Device and Product Engineering fields. I enjoy teaching, working with and training scientists to use different types of analytical instrumentation to solve challenging problems.
View my complete profile
Powered by Blogger.