HPLC CHROMATOGRAPHY HINTS and TIPS FOR CHROMATOGRAPHERS [HPLC TRAINING ARTICLES]

The HPLC "Hints and Tips" found here will be of interest to anyone performing HPLC, UHPLC (UPLC), LC/MS, SFC, CE or GC analysis. Many of these free tips, taken from professional classes that I teach, discuss fundamental to advanced level chromatography concepts in a practical way. Occasionally, I will also include laboratory computer related tips here too. Please "follow" this blog to receive updates of new posts. Hint: Type keywords into the SEARCH bar to quickly find other articles.

Showing posts with label Retention Time. Show all posts
Showing posts with label Retention Time. Show all posts
Saturday, August 21, 2021

Troubleshooting HPLC Gradient Valve / Proportioning Valve / MCGV GPV Leaks. How to Identify Them.

›
HPLC pumps which utilize low-pressure mixing VALVES are known by names such as: "Ternary" (3-solvents) or "Quaternary"...
3 comments:
Saturday, June 2, 2018

Number of theoretical plates (N), Calculation Formulas

›
Number of theoretical plates (N): Often used to quantify the efficiency (performance) of a column (HPLC or GC). "Plates" ...
Saturday, November 28, 2015

HPLC Retention Time Drift, Change, Area Variability or Poor Reproducibility. Common Reasons for it.

›
Retention times and area measurements must be reproducible from run to run. When problems are observed, late, early or variable retention t...
2 comments:
Saturday, June 27, 2015

HPLC K Prime. Also known as: Retention Factor, Capacity Factor): One of the Single Most Important HPLC Parameters of All

›
The role of Capacity Factor / Ratio ( K prime ) in liquid chromatography is to provide a calculation or ratio which defines how much inte...
7 comments:
Friday, April 1, 2011

CHIRAL HPLC / SFC METHOD DEVELOPMENT (Alcohols):

›
We are experts in chiral HPLC and SFC method development of pharmaceutical samples and have operated a contract separations labs for nearly ...
›
Home
View web version

About Me

HPLC EXPERT
AKA: "The HPLC Doctor". An expert Chromatographer, Scientist, Consultant and Inventor who works in the Analytical, BioMedical, BioPharma, Medicinal Chemistry, Medical Device and Product Engineering fields. I enjoy teaching, working with and training scientists to use different types of analytical instrumentation to solve challenging problems.
View my complete profile
Powered by Blogger.