Showing posts with label Peak. Show all posts
Showing posts with label Peak. Show all posts
Saturday, October 19, 2019

HPLC PEAK Fronting and Tailing, Common Reasons For It

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All users of HPLC need to know and be familiar with the correct terms used to describe non-Gaussian shaped peaks. Two of the most common und...
1 comment:
Saturday, December 31, 2016

PEAK PURITY Determination by HPLC Diode Array Chromatography Software (UV/VIS): Limitations and Uses

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" Peak Purity " software determination by HPLC UV/VIS detection is one of the most abused and easily misunderstood features f...
2 comments:
Saturday, September 24, 2016

HPLC Peak Splitting. Common Reasons For It

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True "Split" HPLC peaks, not resulting from co-elution of an other peak, can be caused by a number of chromatography probl...
3 comments:
Saturday, June 27, 2015

HPLC K Prime. Also known as: Retention Factor, Capacity Factor): One of the Single Most Important HPLC Parameters of All

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The role of Capacity Factor / Ratio ( K prime ) in liquid chromatography is to provide a calculation or ratio which defines how much inte...
7 comments:
Saturday, May 30, 2015

HPLC Peak Tailing - Some of the Most Common Reasons For it

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Three easy ways to minimize chromatography peak tailing: (1) Tailing often results from using “Type – A” HPLC silica. Type-A sili...
Saturday, March 29, 2014

External (ESTD) vs. Internal Standard (ISTD) Calibration in HPLC

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Reliable quantitation of sample analytes using HPLC analysis requires accurate and reliable quantitation of a standard(s). For chromatograph...
29 comments:
Saturday, December 21, 2013

Two Common HPLC Problems and their Causes (Sudden changes to either the HPLC Backpressure or Peak Shape)

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   Let's take a quick look at two different problems which you may encounter when operating an HPLC system. We start with the basic obse...
Saturday, August 3, 2013

Proper Wavelength Selection for HPLC Method Development (or Purity Determination)

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Selecting the best HPLC wavelength(s) to monitor during an analysis method for use in quantitation and/or purity determination requires ...
8 comments: