Showing posts with label HPLC. Show all posts
Showing posts with label HPLC. Show all posts
Saturday, April 6, 2024

Troubleshooting a GRADUAL HPLC PUMP PRESSURE INCREASE OVER TIME (When PURGING, DURING an ANALYSIS or when FLOW is DIRECTED TO WASTE)

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A fully equilibrated column at a constant temperature and flow rate should result in a stable back-pressure over time (~1% variation). Have ...
Saturday, July 22, 2023

HPLC Injection Volume: What Should I Dilute It In and How Much Sample Can I Inject?

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HPLC Injection Volume and Solution Tips: For best results, the choice of injection solution and amount must be carefully selected. Successfu...
Saturday, January 29, 2022

Adjusting the HPLC Gradient Time For Changes in Column Diameter and/or Length (same particle size)

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Changes to the column diameter (to scale the method up or down) can be calculated. For an established HPLC method using the same support typ...
Saturday, August 21, 2021

Troubleshooting HPLC Gradient Valve / Proportioning Valve / MCGV GPV Leaks. How to Identify Them.

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HPLC pumps which utilize low-pressure mixing VALVES are known by names such as: "Ternary" (3-solvents) or "Quaternary"...
3 comments:
Saturday, January 9, 2021

Speed Up HPLC Analysis Time Using Higher than "Normal" Flow Rates with SMALLER Particles

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Column efficiency (as described by Van Deemter) in HPLC is largely a function of dispersion, column particle size and the flow rate of the ...
Saturday, September 5, 2020

Tips and Advice for Priming your HPLC PUMP (or similar pumps, FPLC or UHPLC Pump)

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The single most important component of any HPLC system is the Pump module. We often refer to it as "the heart of the HPLC system...
3 comments:
Saturday, April 25, 2020

HPLC Column Cross-Sectional Area and Scaling

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Here is a simple formula to use when scaling up or down Internal Column Diameter to maintain retention values (under constant linear velocit...