HPLC CHROMATOGRAPHY HINTS and TIPS FOR CHROMATOGRAPHERS [HPLC TRAINING ARTICLES]

The HPLC "Hints and Tips" found here will be of interest to anyone performing HPLC, UHPLC (UPLC), LC/MS, SFC, CE or GC analysis. Many of these free tips, taken from professional classes that I teach, discuss fundamental to advanced level chromatography concepts in a practical way. Occasionally, I will also include laboratory computer related tips here too. Please "follow" this blog to receive updates of new posts. Hint: Type keywords into the SEARCH bar to quickly find other articles.

Showing posts with label ESI. Show all posts
Showing posts with label ESI. Show all posts
Saturday, November 3, 2018

HPLC Mobile Phase Composition and LC-MS Electrospray Voltage

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I am often asked about the importance of selecting and optimizing the LC-MS Electrospray Ionization Interface (ESI) voltage. To better under...
Monday, May 21, 2012

Common LC/MS ESI Tune Compounds:

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ION MODE COMPOUND NAME Mw Positive Acetaminophen 151 Positive Caffeine 194 ...
Tuesday, February 21, 2012

Techniques To Enhance Negative Mode ESI in LC/MS

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Many sample types require MS analysis using negative ion electrospray mass spectrometry (ES MS) mode. Sensitivity can be less i...
Thursday, December 1, 2011

Adduct formation in LC-MS Analysis (esp. ESI)

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Almost everything you analyze by Electrospray ionization mass spectrometry will create an adduct with something in the system...
Friday, July 15, 2011

ESI Charge State Calculator Application

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Here is a neat program, created by Robert Winkler, which allows you to calculate the Mw of proteins using data from your electrospray ioniz...
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HPLC EXPERT
AKA: "The HPLC Doctor". An expert Chromatographer, Scientist, Consultant and Inventor who works in the Analytical, BioMedical, BioPharma, Medicinal Chemistry, Medical Device and Product Engineering fields. I enjoy teaching, working with and training scientists to use different types of analytical instrumentation to solve challenging problems.
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