Showing posts with label DAD. Show all posts
Showing posts with label DAD. Show all posts
Saturday, October 6, 2018

HPLC UV - VIS Wavelength Accuracy Check (" Calibration ") Notes

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To verify correct detector wavelength accuracy of your HPLC UV / VIS module it is periodically necessary to measure the wavelength accuracy ...
Saturday, December 31, 2016

PEAK PURITY Determination by HPLC Diode Array Chromatography Software (UV/VIS): Limitations and Uses

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" Peak Purity " software determination by HPLC UV/VIS detection is one of the most abused and easily misunderstood features f...
2 comments:
Saturday, April 2, 2016

Chromophore, Chromophores, UV Absorbing for HPLC Analysis and Detection

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A compound's absorption coefficient relates to its "strength". I find it useful to know which compounds can (and cannot) be ea...
4 comments:
Saturday, March 29, 2014

External (ESTD) vs. Internal Standard (ISTD) Calibration in HPLC

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Reliable quantitation of sample analytes using HPLC analysis requires accurate and reliable quantitation of a standard(s). For chromatograph...
29 comments:
Saturday, November 9, 2013

HPLC Maintenance & Repair Parts To Have on Hand for HPLC Systems

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HPLC (UHPLC) systems are complex instruments which require periodic inspection, cleaning and maintenance. These tasks are critical to mainta...
2 comments:
Saturday, September 7, 2013

Method Development Hint: Use your HPLC Diode Array Detector (DAD or PDA) as a Spectrophotometer

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One of the many useful features of a UV/VIS scanning diode array detector is that it can be employed in flow injection mode to scan a sample...
Saturday, August 3, 2013

Proper Wavelength Selection for HPLC Method Development (or Purity Determination)

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Selecting the best HPLC wavelength(s) to monitor during an analysis method for use in quantitation and/or purity determination requires ...
8 comments:
Saturday, December 8, 2012

Determining the Data Acquisition Rate (Sampling Rate) For Your HPLC Detector

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Another common question I am asked is how to set-up the HPLC detector’s sampling rate. This article is specific to commonly used UV/VIS, not...
3 comments:
Friday, September 30, 2011

UV / VIS, VWD, DAD, PDA HPLC DETECTOR SIGNAL BANDWIDTH (bw) SELECTION

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Modern chromatography UV/VIS detectors offer the operator a choice of one to several hundred different signal wavelength choices (as is th...
10 comments:
Wednesday, March 16, 2011

HPLC Flow Cell Volume & Path Length:

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Modern UV/VIS detectors offer several different flow cell options. The option(s) you select can make a big difference in the level of signa...
1 comment:
Tuesday, March 1, 2011

REFERENCE WAVELENGTHS (as used in HPLC UV/VIS):

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One of the most common problems that I see as a consultant in laboratories which use chromatography for sample analysis relates to how...
12 comments: