HPLC CHROMATOGRAPHY HINTS and TIPS FOR CHROMATOGRAPHERS [HPLC TRAINING ARTICLES]

The HPLC "Hints and Tips" found here will be of interest to anyone performing HPLC, UHPLC (UPLC), LC/MS, SFC, CE or GC analysis. Many of these free tips, taken from professional classes that I teach, discuss fundamental to advanced level chromatography concepts in a practical way. Occasionally, I will also include laboratory computer related tips here too. Please "follow" this blog to receive updates of new posts. Hint: Type keywords into the SEARCH bar to quickly find other articles.

Saturday, March 14, 2020

Changing from Reverse Phase (RP) to Normal Phase (NP) Mode (or vice versa) in your HPLC System

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Two closely related HPLC questions which have the same answer are commonly found in my email folder each week. Both questions deal with conc...
Saturday, February 1, 2020

Air Bubbles Exiting the HPLC PUMP, Reasons Why.

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Reasons For Air Bubbles Exiting The HPLC Pump: Pump Cavitation: When the pump pressure fluctuates wildly up and down, at very low pressu...
Saturday, December 21, 2019

Do your HPLC Methods Meet Good Chromatography Fundamentals? HPLC Training: RETAIN, SEPARATE and RESOLVE

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When an HPLC or LC-MS method is not developed properly, it may not be selective for the sample and may not show any retention on the column...
Saturday, October 19, 2019

HPLC PEAK Fronting and Tailing, Common Reasons For It

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All users of HPLC need to know and be familiar with the correct terms used to describe non-Gaussian shaped peaks. Two of the most common und...
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HPLC EXPERT
AKA: "The HPLC Doctor". An expert Chromatographer, Scientist, Consultant and Inventor who works in the Analytical, BioMedical, BioPharma, Medicinal Chemistry, Medical Device and Product Engineering fields. I enjoy teaching, working with and training scientists to use different types of analytical instrumentation to solve challenging problems.
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