HPLC CHROMATOGRAPHY HINTS and TIPS FOR CHROMATOGRAPHERS [HPLC TRAINING ARTICLES]

The HPLC "Hints and Tips" found here will be of interest to anyone performing HPLC, UHPLC (UPLC), LC/MS, SFC, CE or GC analysis. Many of these free tips, taken from professional classes that I teach, discuss fundamental to advanced level chromatography concepts in a practical way. Occasionally, I will also include laboratory computer related tips here too. Please "follow" this blog to receive updates of new posts. Hint: Type keywords into the SEARCH bar to quickly find other articles.

Saturday, October 19, 2019

HPLC PEAK Fronting and Tailing, Common Reasons For It

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All users of HPLC need to know and be familiar with the correct terms used to describe non-Gaussian shaped peaks. Two of the most common und...
1 comment:
Saturday, September 14, 2019

A Case of Changing Solution pH. Formic Acid Stability in Solution (Methanol)

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Real life examples help to better illustrate problems that I am called in to troubleshoot for clients. As a professional scientific consulta...
2 comments:
Saturday, August 3, 2019

Air Bubbles Exiting the HPLC Vacuum Degasser. Reasons Why

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A common question we are asked to solve relates to why air bubbles might be observed exiting out of an HPLC vacuum degasser module   (where ...
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Saturday, June 29, 2019

Backpressure Changes, Pressure Drop from HPLC Tubing Selection (0.007, 0.005, 0.010")

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In previous articles we have discussed how the choice of column particle size directly changes the system backpressure . Smaller particl...
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HPLC EXPERT
AKA: "The HPLC Doctor". An expert Chromatographer, Scientist, Consultant and Inventor who works in the Analytical, BioMedical, BioPharma, Medicinal Chemistry, Medical Device and Product Engineering fields. I enjoy teaching, working with and training scientists to use different types of analytical instrumentation to solve challenging problems.
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