HPLC CHROMATOGRAPHY HINTS and TIPS FOR CHROMATOGRAPHERS [HPLC TRAINING ARTICLES]

The HPLC "Hints and Tips" found here will be of interest to anyone performing HPLC, UHPLC (UPLC), LC/MS, SFC, CE or GC analysis. Many of these free tips, taken from professional classes that I teach, discuss fundamental to advanced level chromatography concepts in a practical way. Occasionally, I will also include laboratory computer related tips here too. Please "follow" this blog to receive updates of new posts. Hint: Type keywords into the SEARCH bar to quickly find other articles.

Saturday, November 3, 2018

HPLC Mobile Phase Composition and LC-MS Electrospray Voltage

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I am often asked about the importance of selecting and optimizing the LC-MS Electrospray Ionization Interface (ESI) voltage. To better under...
Saturday, October 6, 2018

HPLC UV - VIS Wavelength Accuracy Check (" Calibration ") Notes

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To verify correct detector wavelength accuracy of your HPLC UV / VIS module it is periodically necessary to measure the wavelength accuracy ...
Saturday, August 11, 2018

Cooling Solutions & Mixtures

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An alternative to using an ice bath, peltier cooler or recirculating chiller to cool a solution is to prepare a solution whose freezing poin...
Saturday, July 7, 2018

HPLC Tubing and Fittings; An introduction to Nuts, Ferrules and Tubing Choices

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INTRODUCTION: Setting up a high pressure liquid chromatography (HPLC) system to run trouble-free takes  patience and a strong set of troub...
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HPLC EXPERT
AKA: "The HPLC Doctor". An expert Chromatographer, Scientist, Consultant and Inventor who works in the Analytical, BioMedical, BioPharma, Medicinal Chemistry, Medical Device and Product Engineering fields. I enjoy teaching, working with and training scientists to use different types of analytical instrumentation to solve challenging problems.
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