HPLC CHROMATOGRAPHY HINTS and TIPS FOR CHROMATOGRAPHERS [HPLC TRAINING ARTICLES]

The HPLC "Hints and Tips" found here will be of interest to anyone performing HPLC, UHPLC (UPLC), LC/MS, SFC, CE or GC analysis. Many of these free tips, taken from professional classes that I teach, discuss fundamental to advanced level chromatography concepts in a practical way. Occasionally, I will also include laboratory computer related tips here too. Please "follow" this blog to receive updates of new posts. Hint: Type keywords into the SEARCH bar to quickly find other articles.

Saturday, June 2, 2018

Number of theoretical plates (N), Calculation Formulas

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Number of theoretical plates (N): Often used to quantify the efficiency (performance) of a column (HPLC or GC). "Plates" ...
Saturday, April 21, 2018

The HPLC Restriction Capillary; Troubleshooting, Qualification and Running Without A Column:

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Most types of HPLC pumps will not operate properly without 30 or more bars of back-pressure on their outlets to prevent cavitation and exc...
2 comments:
Saturday, March 10, 2018

Vespel, Tefzel or PEEK Valve Rotor Seals ?

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One of the most common HPLC preventative maintenance parts is the injector valve rotor seal . Worldwide, the majority of these chromatograp...
1 comment:
Saturday, February 10, 2018

HPLC Baseline Stabilization Tips for Refractive Index Detectors (RI or RID)

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If you use refractive index detection (RID) for your HPLC samples, then you are already familiar with the very long equilibration periods ne...
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HPLC EXPERT
AKA: "The HPLC Doctor". An expert Chromatographer, Scientist, Consultant and Inventor who works in the Analytical, BioMedical, BioPharma, Medicinal Chemistry, Medical Device and Product Engineering fields. I enjoy teaching, working with and training scientists to use different types of analytical instrumentation to solve challenging problems.
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