HPLC CHROMATOGRAPHY HINTS and TIPS FOR CHROMATOGRAPHERS [HPLC TRAINING ARTICLES]

The HPLC "Hints and Tips" found here will be of interest to anyone performing HPLC, UHPLC (UPLC), LC/MS, SFC, CE or GC analysis. Many of these free tips, taken from professional classes that I teach, discuss fundamental to advanced level chromatography concepts in a practical way. Occasionally, I will also include laboratory computer related tips here too. Please "follow" this blog to receive updates of new posts. Hint: Type keywords into the SEARCH bar to quickly find other articles.

Saturday, April 8, 2017

LC-MS Contamination? Another Possible Cause. Are your Mobile Phase Bottles and Pick up Filters Clean ?

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One of the mo re common LC/MS problems I am asked to help solve deals with contaminate d LC-MS or LC/MS/MS systems. Over time, m any syst...
Saturday, March 4, 2017

The Three Most Common HPLC Questions and How To Solve Them

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The three most common HPLC related questions I am asked each week can be summarized below. Test your basic chromatography knowledge. Before...
Saturday, February 4, 2017

Determine the HPLC System Dwell Volume (Gradient Delay Volume)

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Note: The total HPLC gradient system dwell volume is different than the HPLC column’s void volume . Two different terms for two very ...
Saturday, December 31, 2016

PEAK PURITY Determination by HPLC Diode Array Chromatography Software (UV/VIS): Limitations and Uses

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" Peak Purity " software determination by HPLC UV/VIS detection is one of the most abused and easily misunderstood features f...
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HPLC EXPERT
AKA: "The HPLC Doctor". An expert Chromatographer, Scientist, Consultant and Inventor who works in the Analytical, BioMedical, BioPharma, Medicinal Chemistry, Medical Device and Product Engineering fields. I enjoy teaching, working with and training scientists to use different types of analytical instrumentation to solve challenging problems.
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