HPLC CHROMATOGRAPHY HINTS and TIPS FOR CHROMATOGRAPHERS [HPLC TRAINING ARTICLES]

The HPLC "Hints and Tips" found here will be of interest to anyone performing HPLC, UHPLC (UPLC), LC/MS, SFC, CE or GC analysis. Many of these free tips, taken from professional classes that I teach, discuss fundamental to advanced level chromatography concepts in a practical way. Occasionally, I will also include laboratory computer related tips here too. Please "follow" this blog to receive updates of new posts. Hint: Type keywords into the SEARCH bar to quickly find other articles.

Saturday, November 26, 2016

HPLC Detector Optical SLIT WIDTH Selection

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A few notes on HPLC Optical Slit Width selection:    Notes:   The chosen slit width setting determines the amount of light which is dir...
Saturday, October 29, 2016

Notes on Cleaning bound Protein from RP HPLC columns:

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First, a few comments: ·          Before proceeding with any column regeneration or cleaning procedures, always refer to the speci...
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Saturday, September 24, 2016

HPLC Peak Splitting. Common Reasons For It

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True "Split" HPLC peaks, not resulting from co-elution of an other peak, can be caused by a number of chromatography probl...
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Saturday, August 27, 2016

Modern HPLC Method Development Tips (PART II):

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This is the second of two articles ( Part I ) which will provide suggestions on how to improve the HPLC (UHPLC) method development process....
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HPLC EXPERT
AKA: "The HPLC Doctor". An expert Chromatographer, Scientist, Consultant and Inventor who works in the Analytical, BioMedical, BioPharma, Medicinal Chemistry, Medical Device and Product Engineering fields. I enjoy teaching, working with and training scientists to use different types of analytical instrumentation to solve challenging problems.
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