HPLC CHROMATOGRAPHY HINTS and TIPS FOR CHROMATOGRAPHERS [HPLC TRAINING ARTICLES]

The HPLC "Hints and Tips" found here will be of interest to anyone performing HPLC, UHPLC (UPLC), LC/MS, SFC, CE or GC analysis. Many of these free tips, taken from professional classes that I teach, discuss fundamental to advanced level chromatography concepts in a practical way. Occasionally, I will also include laboratory computer related tips here too. Please "follow" this blog to receive updates of new posts. Hint: Type keywords into the SEARCH bar to quickly find other articles.

Saturday, January 30, 2016

HPLC Column Support Pore VOLUME

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If an HPLC column had no packing material inside it, then the volume of liquid contained in the cylinder could be calculated using the form...
Saturday, December 26, 2015

Common pKa Values for ACIDS & BASES used in HPLC and LC/MS Method Development

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pKa (25°C)                              ACID 0.3                                           Trifluoroacetic acid 2.15           ...
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Saturday, November 28, 2015

HPLC Retention Time Drift, Change, Area Variability or Poor Reproducibility. Common Reasons for it.

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Retention times and area measurements must be reproducible from run to run. When problems are observed, late, early or variable retention t...
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HPLC EXPERT
AKA: "The HPLC Doctor". An expert Chromatographer, Scientist, Consultant and Inventor who works in the Analytical, BioMedical, BioPharma, Medicinal Chemistry, Medical Device and Product Engineering fields. I enjoy teaching, working with and training scientists to use different types of analytical instrumentation to solve challenging problems.
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