HPLC CHROMATOGRAPHY HINTS and TIPS FOR CHROMATOGRAPHERS [HPLC TRAINING ARTICLES]

The HPLC "Hints and Tips" found here will be of interest to anyone performing HPLC, UHPLC (UPLC), LC/MS, SFC, CE or GC analysis. Many of these free tips, taken from professional classes that I teach, discuss fundamental to advanced level chromatography concepts in a practical way. Occasionally, I will also include laboratory computer related tips here too. Please "follow" this blog to receive updates of new posts. Hint: Type keywords into the SEARCH bar to quickly find other articles.

Saturday, December 26, 2015

Common pKa Values for ACIDS & BASES used in HPLC and LC/MS Method Development

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pKa (25°C)                              ACID 0.3                                           Trifluoroacetic acid 2.15           ...
3 comments:
Saturday, November 28, 2015

HPLC Retention Time Drift, Change, Area Variability or Poor Reproducibility. Common Reasons for it.

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Retention times and area measurements must be reproducible from run to run. When problems are observed, late, early or variable retention t...
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Saturday, October 31, 2015

HPLC System Dead (Dwell) Volume. Is It Static or Can It Change During a Method? Autoinjectors and Gradients.

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I recently read a post on a popular LinkedIn chromatography group where a user asked " if it is possible for the total system volume of...
1 comment:
Saturday, September 26, 2015

Vacuum Pressure Units Conversion Table

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Several of the questions I receive each week by email deal with scientific calculations or conversion of various units . One popular request...
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HPLC EXPERT
AKA: "The HPLC Doctor". An expert Chromatographer, Scientist, Consultant and Inventor who works in the Analytical, BioMedical, BioPharma, Medicinal Chemistry, Medical Device and Product Engineering fields. I enjoy teaching, working with and training scientists to use different types of analytical instrumentation to solve challenging problems.
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