HPLC CHROMATOGRAPHY HINTS and TIPS FOR CHROMATOGRAPHERS [HPLC TRAINING ARTICLES]

The HPLC "Hints and Tips" found here will be of interest to anyone performing HPLC, UHPLC (UPLC), LC/MS, SFC, CE or GC analysis. Many of these free tips, taken from professional classes that I teach, discuss fundamental to advanced level chromatography concepts in a practical way. Occasionally, I will also include laboratory computer related tips here too. Please "follow" this blog to receive updates of new posts. Hint: Type keywords into the SEARCH bar to quickly find other articles.

Saturday, March 29, 2014

External (ESTD) vs. Internal Standard (ISTD) Calibration in HPLC

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Reliable quantitation of sample analytes using HPLC analysis requires accurate and reliable quantitation of a standard(s). For chromatograph...
29 comments:
Saturday, February 22, 2014

More Windows 7 and XP Tips and Tricks

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Transfer Windows Account Settings Utility:  If you are setting up a new PC or just want to transfer your Windows settings from one computer...
Saturday, January 25, 2014

Diagnosing & Troubleshooting HPLC Pressure Fluctuation Problems (Unstable Baseline)

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Few things in chromatography are more frustrating than dealing with large pressure fluctuations (>1% ripple). If the pump pressure is uns...
8 comments:
Saturday, December 21, 2013

Two Common HPLC Problems and their Causes (Sudden changes to either the HPLC Backpressure or Peak Shape)

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   Let's take a quick look at two different problems which you may encounter when operating an HPLC system. We start with the basic obse...
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HPLC EXPERT
AKA: "The HPLC Doctor". An expert Chromatographer, Scientist, Consultant and Inventor who works in the Analytical, BioMedical, BioPharma, Medicinal Chemistry, Medical Device and Product Engineering fields. I enjoy teaching, working with and training scientists to use different types of analytical instrumentation to solve challenging problems.
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