HPLC CHROMATOGRAPHY HINTS and TIPS FOR CHROMATOGRAPHERS [HPLC TRAINING ARTICLES]

The HPLC "Hints and Tips" found here will be of interest to anyone performing HPLC, UHPLC (UPLC), LC/MS, SFC, CE or GC analysis. Many of these free tips, taken from professional classes that I teach, discuss fundamental to advanced level chromatography concepts in a practical way. Occasionally, I will also include laboratory computer related tips here too. Please "follow" this blog to receive updates of new posts. Hint: Type keywords into the SEARCH bar to quickly find other articles.

Saturday, May 18, 2013

HPLC Solution Degassing, Sparging With the Wrong Gas (Gas Choice Matters)

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The other day I took a call from a client whom explained they were having a number of problems with their HPLC pump. They felt that they w...
1 comment:
Saturday, April 13, 2013

Using Smaller Diameter HPLC Columns (Calculate Linear Velocity)

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Lots of 2.1mm ID chromatography columns are appearing on the market right now. Since most of us are using 4.6 mm ID columns to develop HPLC ...
3 comments:
Saturday, March 16, 2013

Introduction to Size Exclusion Chromatography (SEC HPLC)

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Size Exclusion Chromatography . Often k nown as “ SEC ”. Other names used to describe SEC : Gel Filtration Chromatography o...
Tuesday, February 12, 2013

pH Measurement of HPLC Mobile Phase Solutions and Buffers

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Several times each month I am asked how to "correctly check and adjust the pH of an HPLC buffer solution which has an organic solvent c...
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HPLC EXPERT
AKA: "The HPLC Doctor". An expert Chromatographer, Scientist, Consultant and Inventor who works in the Analytical, BioMedical, BioPharma, Medicinal Chemistry, Medical Device and Product Engineering fields. I enjoy teaching, working with and training scientists to use different types of analytical instrumentation to solve challenging problems.
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