HPLC CHROMATOGRAPHY HINTS and TIPS FOR CHROMATOGRAPHERS [HPLC TRAINING ARTICLES]

The HPLC "Hints and Tips" found here will be of interest to anyone performing HPLC, UHPLC (UPLC), LC/MS, SFC, CE or GC analysis. Many of these free tips, taken from professional classes that I teach, discuss fundamental to advanced level chromatography concepts in a practical way. Occasionally, I will also include laboratory computer related tips here too. Please "follow" this blog to receive updates of new posts. Hint: Type keywords into the SEARCH bar to quickly find other articles.

Tuesday, February 12, 2013

pH Measurement of HPLC Mobile Phase Solutions and Buffers

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Several times each month I am asked how to "correctly check and adjust the pH of an HPLC buffer solution which has an organic solvent c...
2 comments:
Friday, January 11, 2013

Common HPLC Calculations:

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Capacity Factor / Retention Factor / Capacity Ratio:  k1 (K Prime) k1 = T(R) - T(0) / T(0) where T(R) equals the retention time of the peak...
3 comments:
Saturday, December 8, 2012

Determining the Data Acquisition Rate (Sampling Rate) For Your HPLC Detector

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Another common question I am asked is how to set-up the HPLC detector’s sampling rate. This article is specific to commonly used UV/VIS, not...
3 comments:
Thursday, October 25, 2012

HPLC Capillary Tubing Connection Volumes:

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The length and internal diameter of the HPLC interconnecting tubing used in your system really does matter. The total volume contained in th...
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HPLC EXPERT
AKA: "The HPLC Doctor". An expert Chromatographer, Scientist, Consultant and Inventor who works in the Analytical, BioMedical, BioPharma, Medicinal Chemistry, Medical Device and Product Engineering fields. I enjoy teaching, working with and training scientists to use different types of analytical instrumentation to solve challenging problems.
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