HPLC CHROMATOGRAPHY HINTS and TIPS FOR CHROMATOGRAPHERS [HPLC TRAINING ARTICLES]

The HPLC "Hints and Tips" found here will be of interest to anyone performing HPLC, UHPLC (UPLC), LC/MS, SFC, CE or GC analysis. Many of these free tips, taken from professional classes that I teach, discuss fundamental to advanced level chromatography concepts in a practical way. Occasionally, I will also include laboratory computer related tips here too. Please "follow" this blog to receive updates of new posts. Hint: Type keywords into the SEARCH bar to quickly find other articles.

Friday, January 11, 2013

Common HPLC Calculations:

›
Capacity Factor / Retention Factor / Capacity Ratio:  k1 (K Prime) k1 = T(R) - T(0) / T(0) where T(R) equals the retention time of the peak...
3 comments:
Saturday, December 8, 2012

Determining the Data Acquisition Rate (Sampling Rate) For Your HPLC Detector

›
Another common question I am asked is how to set-up the HPLC detector’s sampling rate. This article is specific to commonly used UV/VIS, not...
3 comments:
Thursday, October 25, 2012

HPLC Capillary Tubing Connection Volumes:

›
The length and internal diameter of the HPLC interconnecting tubing used in your system really does matter. The total volume contained in th...
Monday, September 24, 2012

HPLC Mobile Phase Filtering & Solvent Inlet Filters

›
HPLC Mobile Phase Filtering:  The tubing and valve passageways of the HPLC system are very narrow and clogs can result from using solu...
‹
›
Home
View web version

About Me

HPLC EXPERT
AKA: "The HPLC Doctor". An expert Chromatographer, Scientist, Consultant and Inventor who works in the Analytical, BioMedical, BioPharma, Medicinal Chemistry, Medical Device and Product Engineering fields. I enjoy teaching, working with and training scientists to use different types of analytical instrumentation to solve challenging problems.
View my complete profile
Powered by Blogger.