HPLC CHROMATOGRAPHY HINTS and TIPS FOR CHROMATOGRAPHERS [HPLC TRAINING ARTICLES]

The HPLC "Hints and Tips" found here will be of interest to anyone performing HPLC, UHPLC (UPLC), LC/MS, SFC, CE or GC analysis. Many of these free tips, taken from professional classes that I teach, discuss fundamental to advanced level chromatography concepts in a practical way. Occasionally, I will also include laboratory computer related tips here too. Please "follow" this blog to receive updates of new posts. Hint: Type keywords into the SEARCH bar to quickly find other articles.

Thursday, December 1, 2011

Adduct formation in LC-MS Analysis (esp. ESI)

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Almost everything you analyze by Electrospray ionization mass spectrometry will create an adduct with something in the system...
Tuesday, November 1, 2011

HPLC Solvent Properties Table (e.g. Polarity, Density, Boiling Point...)

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Here is a link to a table listing common chromatography solvents and their physical properties (e.g. viscosity, polarity, UV absorption...)....
Monday, October 17, 2011

HPLC PUMP SOLVENT COMPRESSIBILITY VALUES

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Have you ever noticed excessive pump ripple (baseline noise) that is not caused by a defective check valve ? The ripple might be due to an ...
Friday, September 30, 2011

UV / VIS, VWD, DAD, PDA HPLC DETECTOR SIGNAL BANDWIDTH (bw) SELECTION

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Modern chromatography UV/VIS detectors offer the operator a choice of one to several hundred different signal wavelength choices (as is th...
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HPLC EXPERT
AKA: "The HPLC Doctor". An expert Chromatographer, Scientist, Consultant and Inventor who works in the Analytical, BioMedical, BioPharma, Medicinal Chemistry, Medical Device and Product Engineering fields. I enjoy teaching, working with and training scientists to use different types of analytical instrumentation to solve challenging problems.
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