HPLC CHROMATOGRAPHY HINTS and TIPS FOR CHROMATOGRAPHERS [HPLC TRAINING ARTICLES]

The HPLC "Hints and Tips" found here will be of interest to anyone performing HPLC, UHPLC (UPLC), LC/MS, SFC, CE or GC analysis. Many of these free tips, taken from professional classes that I teach, discuss fundamental to advanced level chromatography concepts in a practical way. Occasionally, I will also include laboratory computer related tips here too. Please "follow" this blog to receive updates of new posts. Hint: Type keywords into the SEARCH bar to quickly find other articles.

Monday, August 1, 2011

Conversion Factors microgram, nanogram, ppm, ppb and percent:

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Many years ago I made up this handy sample concentration conversion table . Be sure and take into account the density of the compounds firs...
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Friday, July 15, 2011

ESI Charge State Calculator Application

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Here is a neat program, created by Robert Winkler, which allows you to calculate the Mw of proteins using data from your electrospray ioniz...
Monday, July 4, 2011

HPLC to UHPLC Conversion Notes (Column Dimensions, Flow Rate, Injection Volume & System Dispersion)

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The use of ultra-high performance liquid chromatography (UHPLC) columns to reduce analysis times and sometimes improve detection limits is a...
Sunday, June 19, 2011

Some Stereochemistry Terms (Chirality); Structural Isomers, Stereoisomer, Enantiomer, Diastereomer, Meso

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Some of the common terms used in chiral chromatography often need to be reviewed. Here are some of the most common ones we encounter in th...
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HPLC EXPERT
AKA: "The HPLC Doctor". An expert Chromatographer, Scientist, Consultant and Inventor who works in the Analytical, BioMedical, BioPharma, Medicinal Chemistry, Medical Device and Product Engineering fields. I enjoy teaching, working with and training scientists to use different types of analytical instrumentation to solve challenging problems.
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