HPLC CHROMATOGRAPHY HINTS and TIPS FOR CHROMATOGRAPHERS [HPLC TRAINING ARTICLES]

The HPLC "Hints and Tips" found here will be of interest to anyone performing HPLC, UHPLC (UPLC), LC/MS, SFC, CE or GC analysis. Many of these free tips, taken from professional classes that I teach, discuss fundamental to advanced level chromatography concepts in a practical way. Occasionally, I will also include laboratory computer related tips here too. Please "follow" this blog to receive updates of new posts. Hint: Type keywords into the SEARCH bar to quickly find other articles.

Showing posts with label Void. Show all posts
Showing posts with label Void. Show all posts
Saturday, February 4, 2017

Determine the HPLC System Dwell Volume (Gradient Delay Volume)

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Note: The total HPLC gradient system dwell volume is different than the HPLC column’s void volume . Two different terms for two very ...
Saturday, September 24, 2016

HPLC Peak Splitting. Common Reasons For It

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True "Split" HPLC peaks, not resulting from co-elution of an other peak, can be caused by a number of chromatography probl...
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Saturday, January 30, 2016

HPLC Column Support Pore VOLUME

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If an HPLC column had no packing material inside it, then the volume of liquid contained in the cylinder could be calculated using the form...
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HPLC EXPERT
AKA: "The HPLC Doctor". An expert Chromatographer, Scientist, Consultant and Inventor who works in the Analytical, BioMedical, BioPharma, Medicinal Chemistry, Medical Device and Product Engineering fields. I enjoy teaching, working with and training scientists to use different types of analytical instrumentation to solve challenging problems.
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