Showing posts with label UHPLC. Show all posts
Showing posts with label UHPLC. Show all posts
Saturday, January 29, 2022

Adjusting the HPLC Gradient Time For Changes in Column Diameter and/or Length (same particle size)

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Changes to the column diameter (to scale the method up or down) can be calculated. For an established HPLC method using the same support typ...
Saturday, September 5, 2020

Tips and Advice for Priming your HPLC PUMP (or similar pumps, FPLC or UHPLC Pump)

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The single most important component of any HPLC system is the Pump module. We often refer to it as "the heart of the HPLC system...
3 comments:
Saturday, December 21, 2019

Do your HPLC Methods Meet Good Chromatography Fundamentals? HPLC Training: RETAIN, SEPARATE and RESOLVE

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When an HPLC or LC-MS method is not developed properly, it may not be selective for the sample and may not show any retention on the column...
Saturday, September 14, 2019

A Case of Changing Solution pH. Formic Acid Stability in Solution (Methanol)

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Real life examples help to better illustrate problems that I am called in to troubleshoot for clients. As a professional scientific consulta...
2 comments:
Saturday, March 23, 2019

HPLC to UHPLC Conversion Notes (Gradient Time Program Adjustment)

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In an earlier article we discussed how to adjust the flow rate, injection volume and column dimensions when scaling an HPLC method UP or Dow...
Saturday, January 6, 2018

UHPLC TIP: Reducing the Column Temperature to Offset Frictional Heating Effects (Causing Poor Resolution)

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HPLC column temperature is a critical variable that we adjust and optimize during method development. We use it as a variable during the m...
Saturday, April 8, 2017

LC-MS Contamination? Another Possible Cause. Are your Mobile Phase Bottles and Pick up Filters Clean ?

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One of the mo re common LC/MS problems I am asked to help solve deals with contaminate d LC-MS or LC/MS/MS systems. Over time, m any syst...
Saturday, March 4, 2017

The Three Most Common HPLC Questions and How To Solve Them

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The three most common HPLC related questions I am asked each week can be summarized below. Test your basic chromatography knowledge. Before...