Showing posts with label Time. Show all posts
Showing posts with label Time. Show all posts
Saturday, January 29, 2022

Adjusting the HPLC Gradient Time For Changes in Column Diameter and/or Length (same particle size)

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Changes to the column diameter (to scale the method up or down) can be calculated. For an established HPLC method using the same support typ...
Saturday, March 23, 2019

HPLC to UHPLC Conversion Notes (Gradient Time Program Adjustment)

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In an earlier article we discussed how to adjust the flow rate, injection volume and column dimensions when scaling an HPLC method UP or Dow...
Saturday, February 4, 2017

Determine the HPLC System Dwell Volume (Gradient Delay Volume)

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Note: The total HPLC gradient system dwell volume is different than the HPLC column’s void volume . Two different terms for two very ...
Saturday, June 27, 2015

HPLC K Prime. Also known as: Retention Factor, Capacity Factor): One of the Single Most Important HPLC Parameters of All

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The role of Capacity Factor / Ratio ( K prime ) in liquid chromatography is to provide a calculation or ratio which defines how much inte...
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Saturday, December 20, 2014

HPLC Column PORE SIZE (or Pore Diameter) and Retention Time

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Think of your typical porous bare silica support as a big sponge full of holes. All of those holes (pores) are where the sample will migrate...
Sunday, May 1, 2011

Determination of HPLC Column Void Volume / Dead Volume, Dead Time (T zero):

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Column Hold-up Volume, Column Dead Time or ' Column Void Volume ' (the preferred name) are all different terms we apply to find the ...
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