HPLC CHROMATOGRAPHY HINTS and TIPS FOR CHROMATOGRAPHERS [HPLC TRAINING ARTICLES]

The HPLC "Hints and Tips" found here will be of interest to anyone performing HPLC, UHPLC (UPLC), LC/MS, SFC, CE or GC analysis. Many of these free tips, taken from professional classes that I teach, discuss fundamental to advanced level chromatography concepts in a practical way. Occasionally, I will also include laboratory computer related tips here too. Please "follow" this blog to receive updates of new posts. Hint: Type keywords into the SEARCH bar to quickly find other articles.

Showing posts with label Size. Show all posts
Showing posts with label Size. Show all posts
Saturday, April 25, 2020

HPLC Column Cross-Sectional Area and Scaling

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Here is a simple formula to use when scaling up or down Internal Column Diameter to maintain retention values (under constant linear velocit...
Saturday, December 20, 2014

HPLC Column PORE SIZE (or Pore Diameter) and Retention Time

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Think of your typical porous bare silica support as a big sponge full of holes. All of those holes (pores) are where the sample will migrate...
Saturday, October 11, 2014

Appropriate Mixer Volume for HPLC and UHPLC Applications

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For gradient analysis, most analytical scale HPLC (UHPLC) systems incorporate a solvent mixer which is designed to balance the requirements ...
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HPLC EXPERT
AKA: "The HPLC Doctor". An expert Chromatographer, Scientist, Consultant and Inventor who works in the Analytical, BioMedical, BioPharma, Medicinal Chemistry, Medical Device and Product Engineering fields. I enjoy teaching, working with and training scientists to use different types of analytical instrumentation to solve challenging problems.
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