HPLC CHROMATOGRAPHY HINTS and TIPS FOR CHROMATOGRAPHERS [HPLC TRAINING ARTICLES]

The HPLC "Hints and Tips" found here will be of interest to anyone performing HPLC, UHPLC (UPLC), LC/MS, SFC, CE or GC analysis. Many of these free tips, taken from professional classes that I teach, discuss fundamental to advanced level chromatography concepts in a practical way. Occasionally, I will also include laboratory computer related tips here too. Please "follow" this blog to receive updates of new posts. Hint: Type keywords into the SEARCH bar to quickly find other articles.

Showing posts with label Retention. Show all posts
Showing posts with label Retention. Show all posts
Saturday, March 4, 2017

The Three Most Common HPLC Questions and How To Solve Them

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The three most common HPLC related questions I am asked each week can be summarized below. Test your basic chromatography knowledge. Before...
Saturday, February 4, 2017

Determine the HPLC System Dwell Volume (Gradient Delay Volume)

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Note: The total HPLC gradient system dwell volume is different than the HPLC column’s void volume . Two different terms for two very ...
Saturday, December 20, 2014

HPLC Column PORE SIZE (or Pore Diameter) and Retention Time

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Think of your typical porous bare silica support as a big sponge full of holes. All of those holes (pores) are where the sample will migrate...
Tuesday, March 20, 2012

Hydrophilic Interaction Chromatography (HILIC)

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Perhaps you have a polar sample which shows poor or no retention under reverse phase conditions. HILIC may provide you with an alternative...
Sunday, May 1, 2011

Determination of HPLC Column Void Volume / Dead Volume, Dead Time (T zero):

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Column Hold-up Volume, Column Dead Time or ' Column Void Volume ' (the preferred name) are all different terms we apply to find the ...
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HPLC EXPERT
AKA: "The HPLC Doctor". An expert Chromatographer, Scientist, Consultant and Inventor who works in the Analytical, BioMedical, BioPharma, Medicinal Chemistry, Medical Device and Product Engineering fields. I enjoy teaching, working with and training scientists to use different types of analytical instrumentation to solve challenging problems.
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