HPLC CHROMATOGRAPHY HINTS and TIPS FOR CHROMATOGRAPHERS [HPLC TRAINING ARTICLES]

The HPLC "Hints and Tips" found here will be of interest to anyone performing HPLC, UHPLC (UPLC), LC/MS, SFC, CE or GC analysis. Many of these free tips, taken from professional classes that I teach, discuss fundamental to advanced level chromatography concepts in a practical way. Occasionally, I will also include laboratory computer related tips here too. Please "follow" this blog to receive updates of new posts. Hint: Type keywords into the SEARCH bar to quickly find other articles.

Showing posts with label Resolution. Show all posts
Showing posts with label Resolution. Show all posts
Saturday, January 6, 2018

UHPLC TIP: Reducing the Column Temperature to Offset Frictional Heating Effects (Causing Poor Resolution)

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HPLC column temperature is a critical variable that we adjust and optimize during method development. We use it as a variable during the m...
Saturday, February 4, 2017

Determine the HPLC System Dwell Volume (Gradient Delay Volume)

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Note: The total HPLC gradient system dwell volume is different than the HPLC column’s void volume . Two different terms for two very ...
Friday, January 11, 2013

Common HPLC Calculations:

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Capacity Factor / Retention Factor / Capacity Ratio:  k1 (K Prime) k1 = T(R) - T(0) / T(0) where T(R) equals the retention time of the peak...
3 comments:
Wednesday, June 1, 2011

Chiral HPLC and SFC Column Screening Strategies for Method Development:

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We are experts in chiral separations and have learned a great deal about how to efficiently and quickly identify the best conditions t...
1 comment:
Sunday, May 1, 2011

Determination of HPLC Column Void Volume / Dead Volume, Dead Time (T zero):

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Column Hold-up Volume, Column Dead Time or ' Column Void Volume ' (the preferred name) are all different terms we apply to find the ...
18 comments:
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HPLC EXPERT
AKA: "The HPLC Doctor". An expert Chromatographer, Scientist, Consultant and Inventor who works in the Analytical, BioMedical, BioPharma, Medicinal Chemistry, Medical Device and Product Engineering fields. I enjoy teaching, working with and training scientists to use different types of analytical instrumentation to solve challenging problems.
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