HPLC CHROMATOGRAPHY HINTS and TIPS FOR CHROMATOGRAPHERS [HPLC TRAINING ARTICLES]

The HPLC "Hints and Tips" found here will be of interest to anyone performing HPLC, UHPLC (UPLC), LC/MS, SFC, CE or GC analysis. Many of these free tips, taken from professional classes that I teach, discuss fundamental to advanced level chromatography concepts in a practical way. Occasionally, I will also include laboratory computer related tips here too. Please "follow" this blog to receive updates of new posts. Hint: Type keywords into the SEARCH bar to quickly find other articles.

Showing posts with label Integration. Show all posts
Showing posts with label Integration. Show all posts
Saturday, October 19, 2019

HPLC PEAK Fronting and Tailing, Common Reasons For It

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All users of HPLC need to know and be familiar with the correct terms used to describe non-Gaussian shaped peaks. Two of the most common und...
1 comment:
Saturday, September 6, 2014

Common Causes of Baseline Noise in HPLC, UHPLC.

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Achieving a flat baseline which does not exhibit spikes, ghost peaks, drift or wander in an unpredictable manner should be a primary ...
2 comments:
Saturday, March 29, 2014

External (ESTD) vs. Internal Standard (ISTD) Calibration in HPLC

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Reliable quantitation of sample analytes using HPLC analysis requires accurate and reliable quantitation of a standard(s). For chromatograph...
29 comments:
Friday, January 11, 2013

Common HPLC Calculations:

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Capacity Factor / Retention Factor / Capacity Ratio:  k1 (K Prime) k1 = T(R) - T(0) / T(0) where T(R) equals the retention time of the peak...
3 comments:
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HPLC EXPERT
AKA: "The HPLC Doctor". An expert Chromatographer, Scientist, Consultant and Inventor who works in the Analytical, BioMedical, BioPharma, Medicinal Chemistry, Medical Device and Product Engineering fields. I enjoy teaching, working with and training scientists to use different types of analytical instrumentation to solve challenging problems.
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