HPLC CHROMATOGRAPHY HINTS and TIPS FOR CHROMATOGRAPHERS [HPLC TRAINING ARTICLES]

The HPLC "Hints and Tips" found here will be of interest to anyone performing HPLC, UHPLC (UPLC), LC/MS, SFC, CE or GC analysis. Many of these free tips, taken from professional classes that I teach, discuss fundamental to advanced level chromatography concepts in a practical way. Occasionally, I will also include laboratory computer related tips here too. Please "follow" this blog to receive updates of new posts. Hint: Type keywords into the SEARCH bar to quickly find other articles.

Showing posts with label High pressure. Show all posts
Showing posts with label High pressure. Show all posts
Saturday, April 6, 2024

Troubleshooting a GRADUAL HPLC PUMP PRESSURE INCREASE OVER TIME (When PURGING, DURING an ANALYSIS or when FLOW is DIRECTED TO WASTE)

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A fully equilibrated column at a constant temperature and flow rate should result in a stable back-pressure over time (~1% variation). Have ...
Saturday, April 9, 2022

Agilent Quaternary Pump (e.g. G1311A ) "Secret" Operator Tip to FLUSH the HPLC Pump in 1/2 the time!

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One of the most popular " tips " taught in our Agilent 1100 and 1200-series HPLC training classes shows users how to speed up the...
Saturday, August 29, 2015

Terminology. Which is it? "UPLC" (TM) , UHPLC or HPLC? The correct name is still HPLC.

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Proper terminology is very important in science. Brand names or trademarks should not be confused with the names of techniques or methods. I...
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Saturday, October 11, 2014

Appropriate Mixer Volume for HPLC and UHPLC Applications

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For gradient analysis, most analytical scale HPLC (UHPLC) systems incorporate a solvent mixer which is designed to balance the requirements ...
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HPLC EXPERT
AKA: "The HPLC Doctor". An expert Chromatographer, Scientist, Consultant and Inventor who works in the Analytical, BioMedical, BioPharma, Medicinal Chemistry, Medical Device and Product Engineering fields. I enjoy teaching, working with and training scientists to use different types of analytical instrumentation to solve challenging problems.
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