HPLC CHROMATOGRAPHY HINTS and TIPS FOR CHROMATOGRAPHERS [HPLC TRAINING ARTICLES]

The HPLC "Hints and Tips" found here will be of interest to anyone performing HPLC, UHPLC (UPLC), LC/MS, SFC, CE or GC analysis. Many of these free tips, taken from professional classes that I teach, discuss fundamental to advanced level chromatography concepts in a practical way. Occasionally, I will also include laboratory computer related tips here too. Please "follow" this blog to receive updates of new posts. Hint: Type keywords into the SEARCH bar to quickly find other articles.

Showing posts with label Fouling. Show all posts
Showing posts with label Fouling. Show all posts
Saturday, July 22, 2023

HPLC Injection Volume: What Should I Dilute It In and How Much Sample Can I Inject?

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HPLC Injection Volume and Solution Tips: For best results, the choice of injection solution and amount must be carefully selected. Successfu...
Saturday, October 19, 2019

HPLC PEAK Fronting and Tailing, Common Reasons For It

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All users of HPLC need to know and be familiar with the correct terms used to describe non-Gaussian shaped peaks. Two of the most common und...
1 comment:
Saturday, March 4, 2017

The Three Most Common HPLC Questions and How To Solve Them

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The three most common HPLC related questions I am asked each week can be summarized below. Test your basic chromatography knowledge. Before...
Saturday, November 28, 2015

HPLC Retention Time Drift, Change, Area Variability or Poor Reproducibility. Common Reasons for it.

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Retention times and area measurements must be reproducible from run to run. When problems are observed, late, early or variable retention t...
2 comments:
Saturday, December 21, 2013

Two Common HPLC Problems and their Causes (Sudden changes to either the HPLC Backpressure or Peak Shape)

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   Let's take a quick look at two different problems which you may encounter when operating an HPLC system. We start with the basic obse...
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HPLC EXPERT
AKA: "The HPLC Doctor". An expert Chromatographer, Scientist, Consultant and Inventor who works in the Analytical, BioMedical, BioPharma, Medicinal Chemistry, Medical Device and Product Engineering fields. I enjoy teaching, working with and training scientists to use different types of analytical instrumentation to solve challenging problems.
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