Showing posts with label Flow Rate. Show all posts
Showing posts with label Flow Rate. Show all posts
Saturday, April 9, 2022

Agilent Quaternary Pump (e.g. G1311A ) "Secret" Operator Tip to FLUSH the HPLC Pump in 1/2 the time!

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One of the most popular " tips " taught in our Agilent 1100 and 1200-series HPLC training classes shows users how to speed up the...
Saturday, January 29, 2022

Adjusting the HPLC Gradient Time For Changes in Column Diameter and/or Length (same particle size)

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Changes to the column diameter (to scale the method up or down) can be calculated. For an established HPLC method using the same support typ...
Saturday, January 9, 2021

Speed Up HPLC Analysis Time Using Higher than "Normal" Flow Rates with SMALLER Particles

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Column efficiency (as described by Van Deemter) in HPLC is largely a function of dispersion, column particle size and the flow rate of the ...
Saturday, April 25, 2020

HPLC Column Cross-Sectional Area and Scaling

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Here is a simple formula to use when scaling up or down Internal Column Diameter to maintain retention values (under constant linear velocit...
Saturday, February 1, 2020

Air Bubbles Exiting the HPLC PUMP, Reasons Why.

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Reasons For Air Bubbles Exiting The HPLC Pump: Pump Cavitation: When the pump pressure fluctuates wildly up and down, at very low pressu...
Saturday, August 3, 2019

Air Bubbles Exiting the HPLC Vacuum Degasser. Reasons Why

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A common question we are asked to solve relates to why air bubbles might be observed exiting out of an HPLC vacuum degasser module   (where ...
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Saturday, July 12, 2014

USP Guideline Note: HPLC Column Diameter Changes to Maintain Flow Rate Linear Velocity

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USP Allowed Variations in HPLC Column Diameter (*USP 32, Second Supplement, Dec 1, 2009). In the previous USP version, a change of up ...