HPLC CHROMATOGRAPHY HINTS and TIPS FOR CHROMATOGRAPHERS [HPLC TRAINING ARTICLES]

The HPLC "Hints and Tips" found here will be of interest to anyone performing HPLC, UHPLC (UPLC), LC/MS, SFC, CE or GC analysis. Many of these free tips, taken from professional classes that I teach, discuss fundamental to advanced level chromatography concepts in a practical way. Occasionally, I will also include laboratory computer related tips here too. Please "follow" this blog to receive updates of new posts. Hint: Type keywords into the SEARCH bar to quickly find other articles.

Showing posts with label Dead Volume. Show all posts
Showing posts with label Dead Volume. Show all posts
Saturday, July 22, 2023

HPLC Injection Volume: What Should I Dilute It In and How Much Sample Can I Inject?

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HPLC Injection Volume and Solution Tips: For best results, the choice of injection solution and amount must be carefully selected. Successfu...
Saturday, February 4, 2017

Determine the HPLC System Dwell Volume (Gradient Delay Volume)

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Note: The total HPLC gradient system dwell volume is different than the HPLC column’s void volume . Two different terms for two very ...
Saturday, October 31, 2015

HPLC System Dead (Dwell) Volume. Is It Static or Can It Change During a Method? Autoinjectors and Gradients.

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I recently read a post on a popular LinkedIn chromatography group where a user asked " if it is possible for the total system volume of...
1 comment:
Sunday, May 1, 2011

Determination of HPLC Column Void Volume / Dead Volume, Dead Time (T zero):

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Column Hold-up Volume, Column Dead Time or ' Column Void Volume ' (the preferred name) are all different terms we apply to find the ...
18 comments:
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HPLC EXPERT
AKA: "The HPLC Doctor". An expert Chromatographer, Scientist, Consultant and Inventor who works in the Analytical, BioMedical, BioPharma, Medicinal Chemistry, Medical Device and Product Engineering fields. I enjoy teaching, working with and training scientists to use different types of analytical instrumentation to solve challenging problems.
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