Showing posts with label Backpressure. Show all posts
Showing posts with label Backpressure. Show all posts
Saturday, April 6, 2024

Troubleshooting a GRADUAL HPLC PUMP PRESSURE INCREASE OVER TIME (When PURGING, DURING an ANALYSIS or when FLOW is DIRECTED TO WASTE)

›
A fully equilibrated column at a constant temperature and flow rate should result in a stable back-pressure over time (~1% variation). Have ...
Saturday, June 29, 2019

Backpressure Changes, Pressure Drop from HPLC Tubing Selection (0.007, 0.005, 0.010")

›
In previous articles we have discussed how the choice of column particle size directly changes the system backpressure . Smaller particl...
1 comment:
Saturday, April 21, 2018

The HPLC Restriction Capillary; Troubleshooting, Qualification and Running Without A Column:

›
Most types of HPLC pumps will not operate properly without 30 or more bars of back-pressure on their outlets to prevent cavitation and exc...
2 comments:
Saturday, January 6, 2018

UHPLC TIP: Reducing the Column Temperature to Offset Frictional Heating Effects (Causing Poor Resolution)

›
HPLC column temperature is a critical variable that we adjust and optimize during method development. We use it as a variable during the m...
Saturday, May 10, 2014

Gradient Mixing Test For Your HPLC Pump (Step Gradient)

›
The most popular type of gradient pumping module used to perform HPLC analysis utilizes a low pressure mixing valve in their design. These v...
Saturday, December 21, 2013

Two Common HPLC Problems and their Causes (Sudden changes to either the HPLC Backpressure or Peak Shape)

›
   Let's take a quick look at two different problems which you may encounter when operating an HPLC system. We start with the basic obse...