Showing posts with label Back Pressure. Show all posts
Showing posts with label Back Pressure. Show all posts
Saturday, April 6, 2024

Troubleshooting a GRADUAL HPLC PUMP PRESSURE INCREASE OVER TIME (When PURGING, DURING an ANALYSIS or when FLOW is DIRECTED TO WASTE)

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A fully equilibrated column at a constant temperature and flow rate should result in a stable back-pressure over time (~1% variation). Have ...
Saturday, January 9, 2021

Speed Up HPLC Analysis Time Using Higher than "Normal" Flow Rates with SMALLER Particles

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Column efficiency (as described by Van Deemter) in HPLC is largely a function of dispersion, column particle size and the flow rate of the ...
Saturday, June 29, 2019

Backpressure Changes, Pressure Drop from HPLC Tubing Selection (0.007, 0.005, 0.010")

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In previous articles we have discussed how the choice of column particle size directly changes the system backpressure . Smaller particl...
1 comment:
Saturday, April 21, 2018

The HPLC Restriction Capillary; Troubleshooting, Qualification and Running Without A Column:

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Most types of HPLC pumps will not operate properly without 30 or more bars of back-pressure on their outlets to prevent cavitation and exc...
2 comments:
Saturday, January 6, 2018

UHPLC TIP: Reducing the Column Temperature to Offset Frictional Heating Effects (Causing Poor Resolution)

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HPLC column temperature is a critical variable that we adjust and optimize during method development. We use it as a variable during the m...
Saturday, September 2, 2017

HPLC Solvents, Acetonitrile and Methanol, Key Differences and Properties

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Widely used in RP HPLC method development, Acetonitrile (ACN) and Methanol (MeOH) are the two most common solvents you will use with water o...
2 comments:
Saturday, January 25, 2014

Diagnosing & Troubleshooting HPLC Pressure Fluctuation Problems (Unstable Baseline)

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Few things in chromatography are more frustrating than dealing with large pressure fluctuations (>1% ripple). If the pump pressure is uns...
8 comments: