tag:blogger.com,1999:blog-9209375845415652006.post6139787109003575935..comments2024-01-24T09:56:18.495-05:00Comments on HPLC CHROMATOGRAPHY HINTS and TIPS FOR CHROMATOGRAPHERS [HPLC TRAINING ARTICLES] : An Often Ignored HPLC & LC/MS Contamination Source. Did you check your Vacuum Degasser?HPLC EXPERThttp://www.blogger.com/profile/04810178046245936465noreply@blogger.comBlogger2125tag:blogger.com,1999:blog-9209375845415652006.post-87536526622278462292015-08-31T16:12:36.370-04:002015-08-31T16:12:36.370-04:00Big help! We had white crystals forming on the out...Big help! We had white crystals forming on the outlets from our degassing unit going into the pump and did not know what it was. No one every told us we needed to flush the buffers through it. Now we flush the system every day with water as you suggest and it works so much better. Before, we had baseline going all over the place. Some said it was the check valve, but valve was fine. We bypassed the degass and it started working again. So now we flush it with the right liquids and never leave buffers in the system. Works great now for two weeks! Thanks for these great posts. Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-9209375845415652006.post-35616226486313474442015-08-11T16:57:48.850-04:002015-08-11T16:57:48.850-04:00Very good article and a common challenge, especial...Very good article and a common challenge, especially when systems are started up again after a longer period of not using the unit. Iso-propanol (HPLC grade) has proven to be a good cleaning solvent but also storage solvent for vacuum degassers but also the entire system. For normal phase and very small column flows (< 5 uL/min) I would bypass the vaccuum degasser.Rainer Baudernoreply@blogger.com