tag:blogger.com,1999:blog-9209375845415652006.post5089527133826790888..comments2024-01-24T09:56:18.495-05:00Comments on HPLC CHROMATOGRAPHY HINTS and TIPS FOR CHROMATOGRAPHERS [HPLC TRAINING ARTICLES] : Common pKa Values for ACIDS & BASES used in HPLC and LC/MS Method DevelopmentHPLC EXPERThttp://www.blogger.com/profile/04810178046245936465noreply@blogger.comBlogger3125tag:blogger.com,1999:blog-9209375845415652006.post-6490652168023477352018-11-09T16:50:40.682-05:002018-11-09T16:50:40.682-05:00"harm" is not the word I would use. TFA ..."harm" is not the word I would use. TFA is a strong ion pairing agent and may alter the surface chemistry of the HPLC support. In other words, you may not be able to reproduce a different method after using TFA (or any other strong ion pairing agent) on the column. *This is why we dedicate specific columns to specific methods.<br /><br />Good Laboratory Practice: We recommend that you always apply a special label to any column which has been used with an ion paring agent so it does not get used with methods which do not use one. For dedicated sample analysis methods, we always use a dedicated HPLC column. In fact, we maintain different sets of columns which are used with neutral (no ion pairing agents), basic and acidic agents (or modifiers). <br /><br />Columns are low cost consumable items compared to the TIME spent to run the methods on them.HPLC EXPERThttps://www.blogger.com/profile/04810178046245936465noreply@blogger.comtag:blogger.com,1999:blog-9209375845415652006.post-72637308718265262232018-11-07T07:42:05.277-05:002018-11-07T07:42:05.277-05:00Please I need to know if using TFA 0.1% could har...Please I need to know if using TFA 0.1% could harm c18 column as after using it I reinject samples with another method and the second peak appeared after its time by 2 minutes but the first one is in its placeAnonymoushttps://www.blogger.com/profile/17402953915996976439noreply@blogger.comtag:blogger.com,1999:blog-9209375845415652006.post-6450066101336193282018-10-09T07:21:53.841-04:002018-10-09T07:21:53.841-04:00I just liked the article. It was Very refreshing p...I just liked the article. It was Very refreshing post with attractive ideas.It was great to read your blog. Ferrous Sulphatehttps://www.saarthygroup.com/ferrous-sulphatenoreply@blogger.com