tag:blogger.com,1999:blog-9209375845415652006.post4838863383599082009..comments2024-01-24T09:56:18.495-05:00Comments on HPLC CHROMATOGRAPHY HINTS and TIPS FOR CHROMATOGRAPHERS [HPLC TRAINING ARTICLES] : Terminology. Which is it? "UPLC" (TM) , UHPLC or HPLC? The correct name is still HPLC.HPLC EXPERThttp://www.blogger.com/profile/04810178046245936465noreply@blogger.comBlogger3125tag:blogger.com,1999:blog-9209375845415652006.post-88758690785609566892016-09-10T19:30:34.728-04:002016-09-10T19:30:34.728-04:00You would think that scientists would know that th...You would think that scientists would know that the 'U' in UHPLC (or UPLC) was just a marketing campaign to convince users that they needed to buy new HPLC systems with higher max pressure ratings? It seems that some were ignorant and swept up in the sales adverts. We should not call it "UHPLC" at all, but HPLC. All high pressure liquid chromatography is HPLC. Running at higher pressures or using smaller particles would have meant that we would have changed the name everytime we reduced particle size or ran at higher pressures (which we did not do because the name HPLC took care of it). UHPLC is not novel or unique at all. It is still high performance liquid chromatography. HPLC by definition has NEVER specified a specific pressure or particle size. Once we went from "LC" to "HPLC", that name change took care of ALL changes needed. All methods which run at any pressure substantially above gravity flow system (as used in "LC" systems) with packed columns are HPLC methods, not UHPLC or UPLC methods. <br /><br />'HPLC Expert' is correct. Let's stop making up new names for a technique that already has one. To do otherwise makes us scientists look ignorant.Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-9209375845415652006.post-44081299151979322362016-02-25T22:30:57.375-05:002016-02-25T22:30:57.375-05:00I wonder how many high pressure systems (>800 b...I wonder how many high pressure systems (>800 bar) are still running 10-20 minute methods at 'regular hplc' resolutions.Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-9209375845415652006.post-67545147546711035392015-08-31T16:04:18.834-04:002015-08-31T16:04:18.834-04:00Thanks for explaining this. Sounds like vendors ma...Thanks for explaining this. Sounds like vendors made up the name to convince customers that the system they have is no good and they need to buy a more expensive system with a different label on it!Anonymousnoreply@blogger.com