tag:blogger.com,1999:blog-9209375845415652006.post2877613358151621739..comments2024-01-24T09:56:18.495-05:00Comments on HPLC CHROMATOGRAPHY HINTS and TIPS FOR CHROMATOGRAPHERS [HPLC TRAINING ARTICLES] : Notes on Cleaning bound Protein from RP HPLC columns:HPLC EXPERThttp://www.blogger.com/profile/04810178046245936465noreply@blogger.comBlogger3125tag:blogger.com,1999:blog-9209375845415652006.post-16113545440579426752018-12-16T09:52:32.850-05:002018-12-16T09:52:32.850-05:00Good One Good One Umerhttps://www.blogger.com/profile/07185450983811621619noreply@blogger.comtag:blogger.com,1999:blog-9209375845415652006.post-61048059094303167372016-12-02T09:52:05.401-05:002016-12-02T09:52:05.401-05:00Columns are consumable items. At some point you mu...Columns are consumable items. At some point you must decide if the time and materials used (Some solvents are very expensive) to try and clean them are worth the effort. It may not make financial sense to spend days and lots of money trying to clean a column. If the column is marginal at best, that will directly impact your analytical results. Do you want poor quality results which can not be relied upon because you elected to use a contaminated column? <br /><br />Developing high quality methods which fully dissolve all of the samples (at all times), retain, then elute them off the column prevents column fouling. Follow up each analysis method with a separate column wash and re-equilibration method. This should incorporate a stronger solution than your mobile phase (to remove any late eluters). Use freshly prepared, filtered mobile phase solutions. Filter samples. Don't overload the column (do a loading study) with sample. Check the column with a separate std on a regular basis to monitor its efficiency. These types of steps will reduce the need to spend time washing a fouled or contaminated HPLC column.HPLC EXPERThttps://www.blogger.com/profile/04810178046245936465noreply@blogger.comtag:blogger.com,1999:blog-9209375845415652006.post-67915928501932815992016-12-02T09:44:12.990-05:002016-12-02T09:44:12.990-05:00If after trying these wash solutions and the colum...If after trying these wash solutions and the column is still plugged or gives us poor results, should we dispose of the column or continue washing?Anonymousnoreply@blogger.com