- The chosen slit width setting determines the amount of light which is directed to the detector.
- For most HPLC methods, a slit width value of 4 nm is suggested.
- Bandwidth should be set at least as wide as the optical slit width.
Characteristics of Narrow Optical Slit Widths:
- Less light falls on the detector
- Less signal intensity
- Increased baseline noise
- S/N ratio decreases
- Spectral resolution improves which allows for more accurate spectral identification.
- More light falls on the detector
- Greater signal intensity
- Decreased baseline noise
- S/N ratio improves
- Spectral resolution decreases and detail is lost. Less accurate spectral identification and an increase in errors for spectral library matching.