Translator for HPLC HINTS and TIPS for Chromatographers

Saturday, June 14, 2014

Popular LC/MS and HPLC Volatile Mobile Phase Buffers

For applications which utilize an Evaporative Light Scattering Detector (E.L.S.D.), Charged Aerosol Detector (CAD) and/or Mass Spectrometer Detector with Electrospray Ionization source (e.g. LC/MS, MSD or LC/MS/MS), a fully volatile buffering system is usually required. Many of the common HPLC buffers such as sodium or potassium phosphate are not compatible.Use the smallest amount of buffer which provides buffering under the analysis conditions (e.g. 10mM). *Select a buffer which is within 2 pH units (+/- 1) of the sample's pKa and 2 pH units away from any acid's pKa. 

  • For LC/MS applications: Positive ion mode favors acidic mobile phases and Negative ion mode favors basic mobile phases. However, feel free to experiment using both ionization modes and don't forget about using adducts (e.g. ammonium and sodium) with all types of samples to improve signal response. *Maintain these buffers at or below 10 mM. Adjust the pH of the mobile phase to be 1 to 2 units away from your sample's pKa.

Table 1:  Popular examples of useful volatile mobile phase buffering systems.

BUFFERING AGENT                                   USEFUL pH RANGE
  • Ammonium formate                                 2.8 - 4.8; 8.2. - 10.2
  • Formic Acid                                            3.3 - 4.3
  • Pyridine/Formic Acid                               3.3. 4.3, 4.8 - 5.8
  • Trimethylamine/Formic Acid                     3.3 - 4.3, 9.3 - 10.3
  • Ammonium Acetate                                  3.8 - 5.8; 8.2 - 10.2
  • Acetic Acid                                              4.3 - 5.3
  • Trimethylamine/Acetic Acid                      4.3 -5.3, 9.3 - 10.3
  • Ammonia/Formic Acid                              3.3 - 4.3, 8.8 - 9.8
  • Ammonia/Acetic Acid                               4.3 - 5.3, 8.8 - 9.8
  • Ammonium Bicarbonate                           5.9 - 6.9,  8.8 - 9.8
  • Ammonium Carbonate                              5.9 - 6.9, 8.8 - 9.8  
  • 1-Methylpiperidene                                   10.0 - 12.0                              

*Notes: (1) Formic acid is slightly stronger and more volatile than Acetic acid. Formic is often available in higher purity grades and absorbs less in the UV region for most chromatography applications. It works well in positive mode LC/MS analysis, esp at 0.1%. (2) Trifluoroacetic acid (TFA) is volatile, but we do not recommend its use in LC/MS applications as it can raise the background levels in Negative Mode LC/MS (m/z 113) and be very hard to remove from the source and cause contamination.(3) Triethylamine (TEA) causes similar problems with high background signals when used in Positive Mode LC/MS (m/z 102). Many ion pairing reagents suppress ionization.